The Free Thyroxine (T4), Direct test measures the level of unbound T4 hormone in the blood to assess thyroid function. High levels of free T4 are indicative of hyperthyroidism, with symptoms including weight loss, rapid heart rate, and heat intolerance. Low levels of free T4 suggest hypothyroidism, which may cause fatigue, weight gain, and sensitivity to cold. This test is essential for diagnosing thyroid conditions and evaluating the effectiveness of treatment.